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<h1>Practical considerations<aclass="headerlink" href="#practical-considerations" title="Permalink to this heading">#</a></h1>
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<p>We recommend checking out <aclass="reference external" href="https://microtutor.globalbioimaging.org/">Microtutor</a> and <aclass="reference external" href="https://myscope.training/">MyScope</a> for interactive explanations of many of these concepts!</p>
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<h2>Objective selection<aclass="headerlink" href="#objective-selection" title="Permalink to this heading">#</a></h2>
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<p>Microscope objectives have a number of features that must be considered when deciding which objective is right for your experiment</p>
<td><p>Fundamentals of microscopy explained for beginners with lots of images and plain language descriptions of terms used in microscopy</p></td>
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<trclass="row-odd"><td><p>📄 Seeing is believing? A beginners’ guide to practical pitfalls in image acquisition <spanid="id1"><sup><aclass="reference internal" href="../bibliography.html#id101" title="Alison J. North. Seeing is believing? A beginners' guide to practical pitfalls in image acquisition . Journal of Cell Biology, 172(1):9-18, 01 2006. URL: https://doi.org/10.1083/jcb.200507103, doi:10.1083/jcb.200507103.">15</a></sup></span></p></td>
<td><p>A fantastic interactive course on fluorescence microscopy, created by the team at <aclass="reference external" href="https://cite.hms.harvard.edu/">Harvard CITE</a></p></td>
<td><p>An interactive microscope simulator and learning environment developed by MicroscopyAustralia, covering a number of types of microscopy.</p></td>
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<trclass="row-odd"><td><p>📖 Visualizing Cells Through Microscopy</p></td>
<td><p>An open textbook chapter from the “Fundamentals of Cell Biology” book from Oregon State University, a great introductory guide to concepts in microscopy.</p></td>
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<trclass="row-even"><td><p>📄 Seeing is believing? A beginners’ guide to practical pitfalls in image acquisition <spanid="id1"><sup><aclass="reference internal" href="../bibliography.html#id101" title="Alison J. North. Seeing is believing? A beginners' guide to practical pitfalls in image acquisition . Journal of Cell Biology, 172(1):9-18, 01 2006. URL: https://doi.org/10.1083/jcb.200507103, doi:10.1083/jcb.200507103.">15</a></sup></span></p></td>
<td><p>An overview of common concerns to check for during sample preparation and image acquisition</p></td>
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<trclass="row-even"><td><p>📄 Fluorescence microscopy - avoiding the pitfalls <spanid="id2"><sup><aclass="reference internal" href="../bibliography.html#id4" title="Claire M Brown. Fluorescence microscopy–avoiding the pitfalls. J. Cell Sci., 120(Pt 10):1703–1705, May 2007.">16</a></sup></span></p></td>
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<trclass="row-odd"><td><p>📄 Fluorescence microscopy - avoiding the pitfalls <spanid="id2"><sup><aclass="reference internal" href="../bibliography.html#id4" title="Claire M Brown. Fluorescence microscopy–avoiding the pitfalls. J. Cell Sci., 120(Pt 10):1703–1705, May 2007.">16</a></sup></span></p></td>
<td><p>Short overview of some of the most common pitfalls for beginners to fluorescence microscopy</p></td>
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<trclass="row-odd"><td><p>📄 Tutorial: guidance for quantitative confocal microscopy <spanid="id3"><sup><aclass="reference internal" href="../bibliography.html#id81" title="James Jonkman, Claire M Brown, Graham D Wright, Kurt I Anderson, and Alison J North. Tutorial: guidance for quantitative confocal microscopy. Nat. Protoc., 15(5):1585–1611, May 2020.">5</a></sup></span></p></td>
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<trclass="row-even"><td><p>📄 Tutorial: guidance for quantitative confocal microscopy <spanid="id3"><sup><aclass="reference internal" href="../bibliography.html#id81" title="James Jonkman, Claire M Brown, Graham D Wright, Kurt I Anderson, and Alison J North. Tutorial: guidance for quantitative confocal microscopy. Nat. Protoc., 15(5):1585–1611, May 2020.">5</a></sup></span></p></td>
<td><p>Step-by-step guidance on practical considerations for sample preparation, acquisition, and image analysis; primarily though not exclusively aimed at users of confocal microscopy</p></td>
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<trclass="row-even"><td><p>📄 Best practices and tools for reporting reproducible fluorescence microscopy methods <spanid="id4"><sup><aclass="reference internal" href="../bibliography.html#id73" title="Paula Montero Llopis, Rebecca A Senft, Tim J Ross-Elliott, Ryan Stephansky, Daniel P Keeley, Preman Koshar, Guillermo Marqués, Ya-Sheng Gao, Benjamin R Carlson, Thomas Pengo, Mark A Sanders, Lisa A Cameron, and Michelle S Itano. Best practices and tools for reporting reproducible fluorescence microscopy methods. Nat. Methods, 18(12):1463–1476, December 2021.">17</a></sup></span></p></td>
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<trclass="row-odd"><td><p>📄 Best practices and tools for reporting reproducible fluorescence microscopy methods <spanid="id4"><sup><aclass="reference internal" href="../bibliography.html#id73" title="Paula Montero Llopis, Rebecca A Senft, Tim J Ross-Elliott, Ryan Stephansky, Daniel P Keeley, Preman Koshar, Guillermo Marqués, Ya-Sheng Gao, Benjamin R Carlson, Thomas Pengo, Mark A Sanders, Lisa A Cameron, and Michelle S Itano. Best practices and tools for reporting reproducible fluorescence microscopy methods. Nat. Methods, 18(12):1463–1476, December 2021.">17</a></sup></span></p></td>
<td><p>Guidelines and resources for accurate reporting of the most common fluorescence light microscopy techniques, emphasizing the impact of accurate microscopy metadata on data interpretation.’</p></td>
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<td><p>Guidelines and resources for accurate reporting of the most common fluorescence light microscopy techniques, emphasizing the impact of accurate microscopy metadata on data interpretation.</p></td>
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<trclass="row-even"><td><p>📄 Believing is seeing - the deceptive influence of bias in quantitative microscopy<spanid="id5"><sup><aclass="reference internal" href="../bibliography.html#id108" title="Rachel M Lee, Leanna R Eisenman, Satya Khuon, Jesse S Aaron, and Teng-Leong Chew. Believing is seeing - the deceptive influence of bias in quantitative microscopy. J. Cell Sci., January 2024.">18</a></sup></span></p></td>
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