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Description
I am using .pepXML
input and ms2rescore in the commandline.
Despite successfully using the correct self-provided decoy pattern, the percolator
software reports error
Exception caught: ERROR: Reading tab file, error reading in feature vector of PSM mzspec:None:None:scan:2617:FHTLFGIAGLSLLGE/2_4. Check if there are
enough features on this line and if they are all floating point numbers or integers.
I described the None
issue for run_name in a previous ticket, but even if I manually changed the None
to the correct run_name (consistent with file_name), the issue is still not resolved.
The error is reported on the first line of file input. The line has the same number of features as the header line. The last two columns are Peptide
and Proteins
, which contain non-numerical data. I did try to run percolator independently specifying num_features used, however, some other online information says that these two columns are necessary for percolator execution.
Therefore, my final error output is
MS2RescoreError: Number of PSMs in original list (17397) does not match number of PSMs in Percolator
output (0)
any input or solutions?
thanks!