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How to create a pair of primers to amplify a plasmid? #8

@JinyuanSun

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@JinyuanSun

Hello,

I am looking for guidance on how to design a pair of primers to amplify a specific plasmid sequence. I need to ensure that one primer extends downstream (forward primer) and the other extends upstream (reverse primer) from a specified starting position. The primers do not need to be of the same length. Can I achieve this using primers?

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