2xPCR mastermix

Using a PCR mastermix saves time and improves and reproducibility of PCR, especially if many reactions are prepared. In the MEC group,

For a standard PCR reaction of 50 µL, half the volume is 2xPCR mastermix (2xmm). The standard recipe is as follows:

Component	µL	stock	2xmm	final
Water	16	-	-	-
Buffer(xC)	5	10x	2x	1x
MgCl2(mM)	2	50 mM	4 mM	2 mM
dNTPs(mM)	1	10	0.4	0.2
Taq	1	100%	5.0%	2.5%
Total	25	-	-	-

See standard pcr protocol.

The google spreadsheet below contain a recipe.

Create a new tab named after the date of preparation in ISO 8601 format.

Copy paste an old recipe and modify if necessary. You may need to ask for permission to edit.

https://docs.google.com/spreadsheets/d/1KWIgyq6alo-SgLN6TYQieGoHQNPxkIb1-JcRGnsbUoc/edit#gid=849990250