Extraction of chromosomal DNA with PCA (phenol:chloroform:isoamylalcohol)

• Glass beads (~150 µL)

• 150 µL TE 10 X

• 150 µL PCA (phenol : chloroform : isoamyl alcohol at proportion 25:24:1) (Plant lab)

• 20 sec Fast Prep (or 5 min; max rpm; in the LGM disruptor)

• Centrifuge 10 min 14 000 rpm

• DNA is in the upper phase

• Dilute 10x and check in the NANODROP

• Use it as a template.

Reference for this protocol?