SimplyCloning

SimplyCloning

A series of ten youtube videos by Andriy Nemirov describing a sub cloning experiment from planning to verification of the final construct.

Simply Cloning - Chapter 1 - Planning 12:27

<iframe width="640" height="360" src="https://www.youtube.com/embed/rqH5QVFTcJM" frameborder="0" allowfullscreen></iframe>

Simply Cloning - Chapter 2 - PCR 06:39

<iframe width="640" height="360" src="https://www.youtube.com/embed/-jP0r5tPMeE" frameborder="0" allowfullscreen></iframe>

Simply Cloning - Chapter 3 - Vector Restriction Digest 02:52

<iframe width="640" height="360" src="https://www.youtube.com/embed/jhMO7srjhrs" frameborder="0" allowfullscreen></iframe>

Simply Cloning - Chapter 4 - Gel Purification 11:47

<iframe width="640" height="360" src="https://www.youtube.com/embed/97tEvbhvvQg" frameborder="0" allowfullscreen></iframe>

Simply Cloning - Chapter 5 - Insert Restriction Digest 02:55

<iframe width="640" height="360" src="https://www.youtube.com/embed/lMMsbheOnIg" frameborder="0" allowfullscreen></iframe>

Simply Cloning - Chapter 6 - Ligation 06:10

<iframe width="640" height="360" src="https://www.youtube.com/embed/HvHZ0G8nph4" frameborder="0" allowfullscreen></iframe>

Simply Cloning - Chapter 7 - Transformation 08:02

<iframe width="640" height="360" src="https://www.youtube.com/embed/kSyF8JF0Z8o" frameborder="0" allowfullscreen></iframe>

Simply Cloning - Chapter 8 - Plasmid Miniprep 06:05

<iframe width="640" height="360" src="https://www.youtube.com/embed/7PLGDpfdeNc" frameborder="0" allowfullscreen></iframe>

Simply Cloning - Chapter 9 - Clone Verification 05:20

<iframe width="640" height="360" src="https://www.youtube.com/embed/47yJgE8Up9A" frameborder="0" allowfullscreen></iframe>

Simply Cloning - Chapter 10 - Clone Library 02:48

<iframe width="640" height="360" src="https://www.youtube.com/embed/ANzTkdErhQ8" frameborder="0" allowfullscreen></iframe>

Questions

Question 1: What is the cSEGUID of the pSAT6-Bar vector ?

Question 2: Why does he run the gel in Chapter 4 ?