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primers

BjornFJohansson edited this page May 24, 2022 · 5 revisions

PCR primers

We keep PCR primers in this list.

The PCR primer stocks are dissolved to a final concentration of 100 µM when they arrive. This is done by looking at the

Use filtered tips to dissolve the primer stocks in ddH2O.

Never use the primer stocks directly.

Dilute PCR primers to 10 µM working solutions. Use filtered tips when pipetting from primer stocks. Use ddH2O to dilute.

Mark these tubes with the number of the PCR primer.

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