cannot demultiplex #3
Description
Hi, I don't understand what is wrong ...
I have a fastq file with the following sequences. (My barcode is under bracket):
@MN00591:192:000H332YK:1:11102:20930:2477 1:N:0:1
GCCCCCAAAATCAGCGAAATGGGCGCCATTGGTGATGACACCAGGCATCAGAATAAATGTTGTCGGCGAGATATTTGTCGGTCCCAGACTTTCGCTGAACGGCCACGGAGATAGGGCTAGGAGTAACCAGAATGGAGAACGC [ATATCATTAACAGAG] GGTACGCTCATCAGAATTAACC
+
FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF
My barcode file contains the barcode :
#filename,#index1,#index2
A11,ATATCATTAACAGAG,
H10,ATTAATAGTGGTATG,
But the following command keep creating 0 bytes files.
defq -i file.fastq -o demux_out_dir -s index.csv -f .fastq -z 1