Skip to content

Commit d2107e4

Browse files
committed
Version 3.0.0
1 parent 1f8ff79 commit d2107e4

File tree

1 file changed

+7
-3
lines changed

1 file changed

+7
-3
lines changed

README.md

Lines changed: 7 additions & 3 deletions
Original file line numberDiff line numberDiff line change
@@ -40,6 +40,10 @@ not via mail to developers.
4040

4141
Binaries require **SSE4.1 CPU support**; CPUs after 2008 (Penryn) include it.
4242

43+
## Changelog
44+
45+
* 3.0.0: Initial release, included in SMRT Link 6.0.0
46+
4347
## SMRTbell designs
4448

4549
PacBio supports three different SMRTbell designs for IsoSeq libraries.
@@ -171,7 +175,7 @@ Example invocation:
171175
- *ccs*: Get it from the official [SMRT Link](https://www.pacb.com/support/software-downloads/) or compile your own from [unanimity](https://github.com/PacificBiosciences/unanimity)
172176
- *lima*: Pre-compiled binary from [barcoding](https://github.com/pacificbiosciences/barcoding)
173177
- *isoseq3*: Pre-compiled binaries from [releases](https://github.com/PacificBiosciences/IsoSeq3/releases)
174-
178+
175179
Add the directory containing the binaries to `PATH`:
176180

177181
```
@@ -242,14 +246,14 @@ subreads to polished isoforms; timings are system dependent:
242246
user 2832m8.382s
243247
$ ls polished*
244248
polished.bam polished.bam.pbi polished.hq.fasta.gz polished.hq.fastq.gz polished.lq.fasta.gz polished.lq.fastq.gz polished.transcriptset.xml
245-
249+
246250
If you have multiple cells, you should run `--split-bam` in the cluster step which will produce chunked cluster results. Each chunked cluster result can be run as a parallel polish job and merged at the end. The following example splits into 24 chunks. `sample.subreadset.xml` is the dataset containing all the input cells. The `isoseq3 polish` jobs can be run in parallel.
247251

248252
$ isoseq3 cluster demux.primer_5p--primer_3p.bam unpolished.bam --split-bam 24
249253
$ isoseq3 polish unpolished.0.bam sample.subreadset.xml polished.0.bam
250254
$ isoseq3 polish unpolished.1.bam sample.subreadset.xml polished.1.bam
251255
$ ...
252-
256+
253257

254258
## FAQ
255259
### Why IsoSeq3 and not the established IsoSeq1 or IsoSeq2?

0 commit comments

Comments
 (0)