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Binaries require **SSE4.1 CPU support**; CPUs after 2008 (Penryn) include it.
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## Changelog
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* 3.0.0: Initial release, included in SMRT Link 6.0.0
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## SMRTbell designs
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PacBio supports three different SMRTbell designs for IsoSeq libraries.
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-*ccs*: Get it from the official [SMRT Link](https://www.pacb.com/support/software-downloads/) or compile your own from [unanimity](https://github.com/PacificBiosciences/unanimity)
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-*lima*: Pre-compiled binary from [barcoding](https://github.com/pacificbiosciences/barcoding)
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-*isoseq3*: Pre-compiled binaries from [releases](https://github.com/PacificBiosciences/IsoSeq3/releases)
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Add the directory containing the binaries to `PATH`:
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```
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If you have multiple cells, you should run `--split-bam` in the cluster step which will produce chunked cluster results. Each chunked cluster result can be run as a parallel polish job and merged at the end. The following example splits into 24 chunks. `sample.subreadset.xml` is the dataset containing all the input cells. The `isoseq3 polish` jobs can be run in parallel.
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