Skip to content

Duplicated sequences in reference fasta #42

@robbueck

Description

@robbueck

Some taxa have duplicated sequences in the fasta, thus you get an error like this from samtools:

[W::sam_hdr_create] Duplicated sequence "QJKH01000049.1" in file "/tmp/panphlan_wrzoniqg.sam"
[E::sam_hrecs_update_hashes] Duplicate entry "QJKH01000001.1" in sam header
samtools view: failed to add PG line to the header
[W::hts_set_opt] Cannot change block size for this format
samtools sort: failed to read header from "-"
samtools index: "panphlan/output/Dielma_fastidiosa/map_results/SRR14117082_Dielma_fastidiosa_out.bam" is in a format that cannot be usefully indexed
[E] Samtools index encountered some error.

fixed by removing duplicated sequences with seqkit rmdup -n
I had this issue for the genomes of: Cutibacterium_acnes, Roseburia_intestinalis, Olsenella_uli, Acinetobacter_ursingii, Actinomyces_naeslundii, Dialister_pneumosintes, Peptoniphilus_lacrimalis and Dielma_fastidiosa

Metadata

Metadata

Assignees

No one assigned

    Labels

    No labels
    No labels

    Type

    No type

    Projects

    No projects

    Milestone

    No milestone

    Relationships

    None yet

    Development

    No branches or pull requests

    Issue actions