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Hello,
I'm new to the chromstaR package. when I tried to run the Chromstar function I got the following error:
> Chromstar(inputfolder, experiment.table=experiment_table_GMHM,assembly = "mm10",
+ outputfolder=outputfolder, numCPU=4, binsize=1000, stepsize=500,
+ prefit.on.chr=NULL, chromosomes=NULL,
+ mode='combinatorial')
Setting up parallel execution with 4 threads ... 0.8s
================
Binning the data
================
Obtaining chromosome length information from UCSC ... 0s
Error in df[, 2] : object of type 'closure' is not subsettable
I tried using only a subset of my samples, and also tried specifying 'prefit.on.chr='chr12', chromosomes='chr12'',
none of which helped.
I'm using BAM files, not bed.
Can you tell me what I'm doing wrong? any help is much appreciated!
Thanks!
this is my session info:
> sessionInfo()
R version 4.1.3 (2022-03-10)
Platform: x86_64-w64-mingw32/x64 (64-bit)
Running under: Windows 10 x64 (build 22621)
Matrix products: default
locale:
[1] LC_COLLATE=Hebrew_Israel.1255 LC_CTYPE=Hebrew_Israel.1255
[3] LC_MONETARY=Hebrew_Israel.1255 LC_NUMERIC=C
[5] LC_TIME=Hebrew_Israel.1255
attached base packages:
[1] stats4 stats graphics grDevices utils datasets methods base
other attached packages:
[1] chromstaR_1.20.2 chromstaRData_1.20.0 ggplot2_3.4.1
[4] GenomicRanges_1.46.1 GenomeInfoDb_1.30.1 IRanges_2.28.0
[7] S4Vectors_0.32.4 BiocGenerics_0.40.0
loaded via a namespace (and not attached):
[1] SummarizedExperiment_1.24.0 tidyselect_1.2.0
[3] reshape2_1.4.4 lattice_0.20-45
[5] colorspace_2.0-3 vctrs_0.5.2
[7] generics_0.1.3 utf8_1.2.2
[9] rlang_1.0.6 pillar_1.8.1
[11] glue_1.6.2 withr_2.5.0
[13] DBI_1.1.3 BiocParallel_1.28.3
[15] matrixStats_0.63.0 GenomeInfoDbData_1.2.7
[17] foreach_1.5.2 lifecycle_1.0.3
[19] plyr_1.8.8 stringr_1.5.0
[21] zlibbioc_1.40.0 MatrixGenerics_1.6.0
[23] Biostrings_2.62.0 munsell_0.5.0
[25] gtable_0.3.1 mvtnorm_1.1-3
[27] codetools_0.2-19 bamsignals_1.26.0
[29] Biobase_2.54.0 doParallel_1.0.17
[31] parallel_4.1.3 fansi_1.0.3
[33] Rcpp_1.0.9 scales_1.2.1
[35] DelayedArray_0.20.0 XVector_0.34.0
[37] Rsamtools_2.10.0 stringi_1.7.12
[39] dplyr_1.0.10 grid_4.1.3
[41] cli_3.6.0 tools_4.1.3
[43] bitops_1.0-7 magrittr_2.0.3
[45] RCurl_1.98-1.9 tibble_3.1.8
[47] crayon_1.5.2 pkgconfig_2.0.3
[49] Matrix_1.5-3 assertthat_0.2.1
[51] rstudioapi_0.14 iterators_1.0.14
[53] R6_2.5.1 GenomicAlignments_1.30.0
[55] compiler_4.1.3
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