BOSS-RUNS for barcode balancing

[ganschow]

Hi,
Would it be possible to use BOSS-RUNS for barcode balancing with custom barcodes? Like specifying the barcodes (or adapter + barcode seqs) as targets and the tool will mitigate coverage bias between the barcoded samples?

[W-L]

Hi,
Apologies for the very late reply! Unfortunately, this functionality is currently not implemented. Due to technical reasons reference sequences need to be of a certain minimum length (longer than typical nanopore reads). Otherwise internal calculations lead to unexpected results. For now, this is still on our todo list.
Thanks and best wishes