diff --git a/Testing/Data/SystemTest/ZOOM00038238.nxs.md5 b/Testing/Data/SystemTest/ZOOM00038238.nxs.md5
new file mode 100644
index 000000000000..8f272906a84a
--- /dev/null
+++ b/Testing/Data/SystemTest/ZOOM00038238.nxs.md5
@@ -0,0 +1 @@
+f46b506522760c88b9a7baec5ffbcb03
diff --git a/Testing/Data/SystemTest/ZOOM00038335.nxs.md5 b/Testing/Data/SystemTest/ZOOM00038335.nxs.md5
new file mode 100644
index 000000000000..8ab856cb02b5
--- /dev/null
+++ b/Testing/Data/SystemTest/ZOOM00038335.nxs.md5
@@ -0,0 +1 @@
+8dc37e0a56316abf322b851c04f64361
diff --git a/Testing/SystemTests/tests/framework/PolarizationCorrections/DepolarizedAnalyserTransmissionTest.py b/Testing/SystemTests/tests/framework/PolarizationCorrections/DepolarizedAnalyserTransmissionTest.py
new file mode 100644
index 000000000000..7e369b0d3b5d
--- /dev/null
+++ b/Testing/SystemTests/tests/framework/PolarizationCorrections/DepolarizedAnalyserTransmissionTest.py
@@ -0,0 +1,63 @@
+# Mantid Repository : https://github.com/mantidproject/mantid
+#
+# Copyright © 2024 ISIS Rutherford Appleton Laboratory UKRI,
+#   NScD Oak Ridge National Laboratory, European Spallation Source,
+#   Institut Laue - Langevin & CSNS, Institute of High Energy Physics, CAS
+# SPDX - License - Identifier: GPL - 3.0 +
+
+import systemtesting
+
+from mantid.api import AnalysisDataService
+from mantid.simpleapi import *
+
+
+class DepolarizedAnalyzerTransmissionTest(systemtesting.MantidSystemTest):
+    def runTest(self):
+        Load("ZOOM00038238.nxs", OutputWorkspace="mt_run")
+        Load("ZOOM00038335.nxs", OutputWorkspace="dep_run")
+
+        mt_group = self._prepare_workspace("mt_run")
+        dep_group = self._prepare_workspace("dep_run")
+        mt = self._average_workspaces_in_group(list(mt_group))
+        dep = self._average_workspaces_in_group(list(dep_group))
+
+        DepolarizedAnalyserTransmission(dep, mt, OutputWorkspace="params" "", OutputFitCurves="curves")
+
+    def _prepare_workspace(self, input_ws_name):
+        converted = ConvertUnits(input_ws_name, "Wavelength", AlignBins=True, StoreInADS=False)
+        monitor_3 = CropWorkspace(converted, StartWorkspaceIndex=2, EndWorkspaceIndex=2, StoreInADS=False)
+        monitor_4 = CropWorkspace(converted, StartWorkspaceIndex=3, EndWorkspaceIndex=3, StoreInADS=False)
+        return monitor_4 / monitor_3
+
+    def _average_workspaces_in_group(self, ws_list):
+        return sum(ws_list) / 4
+
+    def validate(self):
+        self.tolerance = 1e-5
+        result_curves = "curves"
+        reference_curves = "DepolCurvesReference.nxs"
+        result_params = "params"
+        reference_params = "DepolParamsReference.nxs"
+
+        def validate_group(result, reference):
+            Load(Filename=reference, OutputWorkspace=reference)
+            compare_alg = AlgorithmManager.create("CompareWorkspaces")
+            compare_alg.setPropertyValue("Workspace1", result)
+            compare_alg.setPropertyValue("Workspace2", reference)
+            compare_alg.setPropertyValue("Tolerance", str(self.tolerance))
+            compare_alg.setChild(True)
+
+            compare_alg.execute()
+            if compare_alg.getPropertyValue("Result") != "1":
+                print("Workspaces do not match.")
+                print(self.__class__.__name__)
+                SaveNexus(InputWorkspace=result, Filename=f"{self.__class__.__name__}-{result}-mismatch.nxs")
+                return False
+            return True
+
+        is_curves_match = validate_group(result_curves, reference_curves)
+        is_params_match = validate_group(result_params, reference_params)
+        return is_curves_match and is_params_match
+
+    def cleanup(self):
+        AnalysisDataService.clear()
diff --git a/Testing/SystemTests/tests/framework/reference/DepolCurvesReference.nxs.md5 b/Testing/SystemTests/tests/framework/reference/DepolCurvesReference.nxs.md5
new file mode 100644
index 000000000000..166923be1775
--- /dev/null
+++ b/Testing/SystemTests/tests/framework/reference/DepolCurvesReference.nxs.md5
@@ -0,0 +1 @@
+e508561a750eb6064ff40f11fba8d31b
diff --git a/Testing/SystemTests/tests/framework/reference/DepolParamsReference.nxs.md5 b/Testing/SystemTests/tests/framework/reference/DepolParamsReference.nxs.md5
new file mode 100644
index 000000000000..2243eed1db00
--- /dev/null
+++ b/Testing/SystemTests/tests/framework/reference/DepolParamsReference.nxs.md5
@@ -0,0 +1 @@
+45c39b9e17ab1978975817bc3a296e39