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The following lines of code cause "Error in .rowNamesDF<-(x, value = value) :
duplicate 'row.names' are not allowed." As far as I can see, these lines are not particularly essential to the functioning of the script. Do you foresee any problems if I run the script with these lines commented out?
message("processing flags") ## We will process the flags in R ## (an alternative is multiple scanBam calls...) bam$flag <- bam$flag %% 256
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