diff --git a/pyproject.toml b/pyproject.toml
index d6ba8117..dc388cde 100644
--- a/pyproject.toml
+++ b/pyproject.toml
@@ -46,6 +46,17 @@ vcf2zarr = "bio2zarr.cli:vcf2zarr"
 plink2zarr = "bio2zarr.cli:plink2zarr"
 vcf_partition = "bio2zarr.cli:vcf_partition"
 
+[project.optional-dependencies]
+dev = [
+  "msprime",
+  "pysam",
+  "pytest",
+  "pytest-coverage",
+  "pytest-xdist",
+  "sgkit",
+  "tqdm"
+]
+
 [tool.setuptools]
 packages = ["bio2zarr"]
 
diff --git a/requirements/development.txt b/requirements/development.txt
deleted file mode 100644
index 83a73527..00000000
--- a/requirements/development.txt
+++ /dev/null
@@ -1,12 +0,0 @@
-bed_reader
-click
-cyvcf2
-msprime
-pysam
-pytest
-pytest-coverage
-pytest-xdist
-sgkit
-tabulate
-tqdm
-zarr>=2.17