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Description
I tried it with the ensemble annotation and I received the following error:
Executing the following command: iCount segment Homo_sapiens.GRCh38.93.gtf Homo_sapiens.GRCh38.93.seg.gtf GRCh38.p7.genome.fa.fai
Input parameters for function 'get_segments' in iCount.genomes.segment
annotation: Homo_sapiens.GRCh38.93.gtf
segmentation: Homo_sapiens.GRCh38.93.seg.gtf
fai: GRCh38.p7.genome.fa.fai
report_progress: False
Calculating intergenic intervals...
Segmentation stored in Homo_sapiens.GRCh38.93.seg.gtf
Making also gene level segmentation...
[FileNotFoundError] 2
File "/home/icuser/iCount_src/iCount/cli.py", line 439, in main
result_object = func(**args)
File "/home/icuser/iCount_src/iCount/genomes/segment.py", line 1037, in get_segments
make_regions(segmentation, out_dir=os.path.dirname(segmentation))
File "/home/icuser/iCount_src/iCount/genomes/segment.py", line 357, in make_regions
os.makedirs(out_dir, exist_ok=True)
File "/home/icuser/.icountenv/lib/python3.4/os.py", line 237, in makedirs
mkdir(name, mode)