Home page for NOAA 'Omics Github repository

Water collection and filtration protocol using sterivex filters to collect envirnomental DNA from marine ecosystems. This collection and filtration protocol is used by the NOAA PMEL Ocean Molecular Ecology (OME) Group.

Nucleic acids extraction from 47 mm disc filters using the Qiagen DNeasy Blood and Tissue Kit with some modifications to the manufacturer’s protocol. These extraction protocols, adapted from Spens et al 2017, are used by the NOAA PMEL Ocean Molecular Ecology (OME) Group.

This PCR protocol is for amplifying the Small subunit ribosomal ribonucleic acid (SSU rRNA) 18S v9 gene in eukaryotes.

This protocol is for quantifying the concentration of DNA in the product of a DNA extraction.

This PCR protocol is for amplifying the mitochondrial displacement loop (D-loop) gene also known as the mitochondial control region in marine mammals.

This protocol is for running and imaging PCR product using gel electrophoresis.

This PCR protocol is for amplifying the 16S rRNA v4-v5 region.

This PCR protocol is for amplifying the 16S large subunit ribosomal RNA mitochondrial gene in eukaryotes.

This PCR protocol is for amplifying the internal transcribed spacer 1 (ITS1) gene in eukaryotes, specifically Pseudo-nitzschia spp. diatoms

Nucleic acids extraction from the sterivex filters using the Qiagen DNeasy Blood and Tissue Kit with some modifications to the manufacturer’s protocol. These extraction protocols, adapted from Spens et al 2017, are used by the NOAA PMEL Ocean Molecular Ecology (OME) Group.

This PCR protocol is for amplifying the 12S mitochondrial ribosomal RNA gene in vertebrates.

This PCR protocol is for amplifying the Small subunit ribosomal ribonucleic acid (SSU rRNA) 18S v4 gene in eukaryotes.

This PCR protocol is for amplifying the cytochrome c oxidase subunit I (COI) mitochondrial gene in eukaryotes.

This PCR protocol is for amplifying This protocol is for amplifying the small subunit ribosomal ribonucleic acid (SSU rRNA) 18S v8 gene in eukaryotes.

Nucleic acids extraction from the sterivex filters using the Qiagen DNeasy Blood and Tissue Kit with some modifications to the manufacturer’s protocol, including the use of a QIAvac. These extraction protocols, adapted from Spens et al 2017, are used by the NOAA PMEL Ocean Molecular Ecology (OME) Group.