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Water collection and filtration protocol using sterivex filters to collect envirnomental DNA from marine ecosystems. This collection and filtration protocol is used by the NOAA PMEL Ocean Molecular Ecology (OME) Group.
Protocols used by AOML Omics labs, following the BeBOP-OBON standard
This protocol is for running and imaging PCR product using gel electrophoresis.
This PCR protocol is for amplifying the Small subunit ribosomal ribonucleic acid (SSU rRNA) 18S v9 gene in eukaryotes.
This PCR protocol is for amplifying the 16S rRNA v4-v5 region.
This protocol is for quantifying the concentration of DNA in the product of a DNA extraction.
This PCR protocol is for amplifying the mitochondrial displacement loop (D-loop) gene also known as the mitochondial control region in marine mammals.
Protocol for extraction of DNA from Sterivex filters using a KingFisher robot (BeBOP format)
Protocol for sampling water using Sterivex with Zymo ZR beads and DNA/RNA Shield
Protocol for extracting DNA from sediment trap samples using the Qiagen PowerSoil Pro Kit
Protocol for preparing 16S V4-V5 (EMP) libraries for Michigan State RTSF
Protocol for preparing 18S V9 (EMP) libraries for Michigan State RTSF
This PCR protocol is for amplifying the internal transcribed spacer 1 (ITS1) gene in eukaryotes, specifically Pseudo-nitzschia spp. diatoms
Protocol for sampling water using Sterivex with no ZR beads or preservative (dry)
Protocol for preparing 12S V5-V6 (MiFish) libraries for Michigan State RTSF
Nucleic acids extraction from the sterivex filters using the Qiagen DNeasy Blood and Tissue Kit with some modifications to the manufacturer’s protocol. These extraction protocols, adapted from Spens et al 2017, are used by the NOAA PMEL Ocean Molecular Ecology (OME) Group.
This PCR protocol is for amplifying the 12S mitochondrial ribosomal RNA gene in vertebrates.
Protocol for preparing metagenomic libraries using Illumina DNA Prep
This PCR protocol is for amplifying the 16S large subunit ribosomal RNA mitochondrial gene in eukaryotes.
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